Ruoslahti Research Laboratories at UC Santa Barbara and Sanford-Burnham
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Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule...

Pierschbacher, Md Hayman, Eg Erkki Ruoslahti

Published in Cell

Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-promoting, chymotryptic, 120 kil...

Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor

R, Pytela Md, Pierschbacher Erkki Ruoslahti

Published in Cell

Affinity chromatography was used to identify a putative cell surface receptor for fibronectin. A large cell-attachment-promoting fibronectin fragment was used as the affinity matrix, and specific elution was effected by using synthetic peptides containing the sequence Arg-Gly-Asp, which is derived from the cell recognition sequence in the fibronect...

Anchorage dependence, integrins, and apoptosis.

Erkki Ruoslahti Reed, Jc

Published in Cell

Fibulin, a novel protein that interacts with the fibronectin receptor beta subunit cytoplasmic domain.

Argraves, Ws Dickerson, K Burgess, Wh Erkki Ruoslahti

Published in Cell

A 100 kd protein was isolated from tissue and cell extracts by affinity chromatography on a synthetic peptide representing the cytoplasmic domain of the fibronectin receptor beta subunit. The 100 kd protein also bound to native fibronectin receptor, and this binding could be reversed with EDTA. Calcium may be the divalent cation required for the bi...

Integrin activation by R-ras.

Zhang, Z Vuori, K Wang, H Reed, Jc Erkki Ruoslahti

Published in Cell

Expression of a constitutively active R-ras converted two cell lines that grow in suspension into highly adherent cells. There was little change in cell surface expression of integrins, but attachment to surfaces coated with the integrin ligands was greatly enhanced. Cells transfected with activated R-ras bound integrin ligands from solution with h...

Nonhelical, fibronectin-binding basement-membrane collagen from endodermal cell culture.

Engvall, E Bell, Ml Carlsson, Rn Miller, Ej Erkki Ruoslahti

Published in Cell

A novel method of affinity chromatography on insolubilized collagen-binding fragments of fibronectin was utilized to isolate a random-coil collagenous protein from culture media of mouse teratocarcinoma-derived endodermal cells. These cells also produced another collagenous protein, which did not bind to fibronectin but could be isolated by differe...

A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors.

Jan, Y Matter, M Pai, Jt Chen, Yl Pilch, J Komatsu, M Ong, E Fukuda, M Erkki Ruoslahti

Published in Cell

A delicate balance of signals regulates cell survival. One set of these signals is derived from integrin-mediated cell adhesion to the extracellular matrix (ECM). Loss of cell attachment to the ECM causes apoptosis, a process known as anoikis. In searching for proteins involved in cell adhesion-dependent regulation of anoikis, we identified Bit1, a...

Arg-Gly-Asp: a versatile cell recognition signal.

Erkki Ruoslahti Pierschbacher, Md

Published in Cell

Proteoglycans as modulators of growth factor activities.

Erkki Ruoslahti Yamaguchi, Y

Published in Cell

Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary c...

Giancotti, Fg Erkki Ruoslahti

Published in Cell

We report here on gene transfer studies designed to investigate the function of the alpha 5 beta 1 integrin and its role in transformation. Transfection of the human alpha 5 and beta 1 cDNAs into transformed Chinese hamster ovary (CHO) cells followed by methotrexate-induced amplification yielded clonal cell lines overexpression this fibronectin rec...

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