Oldberg, A Hayman, Eg Erkki Ruoslahti
Published in
Journal of Biological Chemistry
A proteoglycan was isolated from ascites fluid produced by a rat yolk sac tumor. The glycosaminoglycan chains of the proteoglycan are all sensitive to digestion with chondroitinase ABC and about 90% are sensitive to chondroitinase AC. The proteoglycan contains 5% protein. Amino acid analysis revealed a high content of serine and glycine which toget...
Hsia, Jc Er, Ss Tan, Ct Estes, T Erkki Ruoslahti
Published in
Journal of Biological Chemistry
A dianionic spin label, 1-L-glutamate-5-N-(1-oxyl-2,2,6,6-tetramethyl-4-aminopiperidinyl)-i,4-dinitrobenzene, has been used to probe the relative binding specificity of a single anionic ligand site on bovine alpha-fetoprotein (AFP) to arachidonate, bilirubin, docosahexaenoate, and plamitate. The binding isotherm of the spin label with AFP, as shown...
Zou, Jx Liu, Y Pasquale, Eb Erkki Ruoslahti
Published in
Journal of Biological Chemistry
One of the prominent effects of the Src kinase is to reduce cell adhesion. The small GTPase, R-Ras, affects cell adhesion by maintaining integrin activity, and the ability of R-Ras to do so can be regulated by phosphorylation of a tyrosine residue located in its effector domain by an Eph receptor kinase (Zou, J. X., Wang, B., Kalo, M. S., Zisch, A....
Birkenmeier, Tm Mcquillan, Jj Boedeker, Ed Argraves, Ws Erkki Ruoslahti Dean, Dc
Published in
Journal of Biological Chemistry
A genomic clone containing the gene for the alpha 5 subunit of the human alpha 5 beta 1 integrin complex was isolated by screening a human genomic library with the previously described alpha 5 cDNA (Argraves, W. S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M. D., and Ruoslahti, E. (1987) J. Cell Biol 105, 1183-1190). The alpha 5 gene 5 -f...
Erkki Ruoslahti Hayman, Eg Kuusela, P Shively, Je Engvall, E
Published in
Journal of Biological Chemistry
Borges, E Jan, Y Erkki Ruoslahti
Published in
Journal of Biological Chemistry
Integrin-mediated cell attachment and growth factor stimulation often act synergistically on cell proliferation, differentiation, migration, and survival. Some of these synergistic effects depend on the physical interaction of integrins with growth factor receptors. Here we examine the nature of the physical interaction between the alpha(v)beta(3) ...
Suzuki, S Pierschbacher, Md Hayman, Eg Nguyen, K Ohgren, Y Erkki Ruoslahti
Published in
Journal of Biological Chemistry
The structure of vitronectin, an adhesive protein isolated from human plasma, was studied by chemical fragmentation. Partial cleavage of vitronectin with cyanogen bromide in 70% formic acid generated four main fragments with masses of 53,000, 43,000, 35,000, and 12,000 daltons arising from both the 75- and 65-kDa vitronectin polypeptides and a 10-k...
Wang, B Zou, Jx Ek-Rylander, B Erkki Ruoslahti
Published in
Journal of Biological Chemistry
R-Ras contains a proline-rich motif that resembles SH3 domain-binding sites but that has escaped notice previously. We show here that this site in R-Ras is capable of binding SH3 domains and that the SH3 domain binding may be important for R-Ras function. A fusion protein containing the SH3 domains of the adaptor protein Nck interacted strongly wit...
Kirchhofer, D Gailit, J Erkki Ruoslahti Grzesiak, J Pierschbacher, Md
Published in
Journal of Biological Chemistry
The presence of manganese (Mn2+) significantly increases the binding of the platelet surface receptor GPIIb/IIIa to two synthetic peptides Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) and Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-Asp-Val (L10) that contain the recognition sequences RGD and KQAGDV, respectively. This results in an increase in the amount of GPIIb/IIIa ads...
H, Biliran Y, Jan R, Chen Eb, Pasquale E, Ruoslahti
Published in
Journal of Biological Chemistry
Bit1 (Bcl-2 inhibitor of transcription) is a mitochondrial protein that induces caspase-independent apoptosis upon its release into the cytoplasm. Bit1 is primarily associated with anoikis (cell death induced by detachment from the extracellular matrix), because the apoptotic function of Bit1 is inhibited by integrin-mediated cell attachment but no...
