Aspberg, A Miura, R Bourdoulous, S Shimonaka, M Heinegârd, D Schachner, M Erkki Ruoslahti Yamaguchi, Y
Published in
Proceedings of the National Academy of Sciences
The lecticans are a family of chondroitin sulfate proteoglycans including aggrecan, versican, neurocan, and brevican. The C-terminal globular domains of lecticans are structurally related to selectins, consisting of a C-type lectin domain flanked by epidermal growth factor and complement regulatory protein domains. The C-type lectin domain of versi...
Bourdon, Ma Oldberg, A Pierschbacher, M Erkki Ruoslahti
Published in
Proceedings of the National Academy of Sciences
We report the identification and DNA sequence of a chondroitin sulfate proteoglycan core protein cDNA. A cDNA clone, pPG1, was selected from a rat yolk sac tumor poly(A)+RNA-derived cDNA library by using synthetic oligonucleotides predicted from the NH2-terminal peptide sequence of the mature chondroitin sulfate proteoglycan. The resulting sequence...
Porkka, K Laakkonen, P Hoffman, Ja Bernasconi, M Erkki Ruoslahti
Published in
Proceedings of the National Academy of Sciences
We used a screening procedure to identify protein domains from phage-displayed cDNA libraries that bind both to bone marrow endothelial progenitor cells and tumor vasculature. Screening phage for binding of progenitor cell-enriched bone marrow cells in vitro, and for homing to HL-60 human leukemia cell xenograft tumors in vivo, yielded a cDNA fragm...
Hamzah, J V. Ramana Kotamraju Seo, Jw Lilach Agemy Valentina Fogal Mahakian, Lm Peters, D Lise Roth Gagnon, Mk Ferrara, Kw
...
Published in
Proceedings of the National Academy of Sciences
The ability to selectively deliver compounds into atherosclerotic plaques would greatly benefit the detection and treatment of atherosclerotic disease. We describe such a delivery system based on a 9-amino acid cyclic peptide, LyP-1. LyP-1 was originally identified as a tumor-homing peptide that specifically recognizes tumor cells, tumor lymphatics...
Peters, David Kastantin, Mark Kotamraju, Venkata Ramana Karmali, Priya P. Gujraty, Kunal Tirrell, Matthew Ruoslahti, Erkki
Published in
Proceedings of the National Academy of Sciences of the United States of America
Subtle clotting that occurs on the luminal surface of atherosclerotic plaques presents a novel target for nanoparticle-based diagnostics and therapeutics. We have developed modular multifunctional micelles that contain a targeting element, a fluorophore, and, when desired, a drug component in the same particle. Targeting atherosclerotic plaques in ...
Jh, Park G, Von Maltzahn Mj, Xu V, Fogal Vr, Kotamraju E, Ruoslahti Sn, Bhatia Mj, Sailor
Published in
Proceedings of the National Academy of Sciences
A significant barrier to the clinical translation of systemically administered therapeutic nanoparticles is their tendency to be removed from circulation by the mononuclear phagocyte system. The addition of a targeting ligand that selectively interacts with cancer cells can improve the therapeutic efficacy of nanomaterials, although these systems h...
Quaroni, A Isselbacher, Kj Erkki Ruoslahti
Published in
Proceedings of the National Academy of Sciences
Synthesis of fibronectin in an epithelial cell line (IEC-6) established from rat small intestine was demonstrated by using immunofluorescence, radioimmunoassay, and collagen-binding. Internally labeled radioactive fibronectin isolated from the IEC-6 cells gave a single main band in sodium dodecyl sulfate/polyacrylamide gel electrophoresis under red...
Pytela, R Pierschbacher, Md Erkki Ruoslahti
Published in
Proceedings of the National Academy of Sciences
Affinity chromatography was used to identify a cell surface receptor for the adhesive protein vitronectin. Detergent extracts of human osteosarcoma (MG-63) cells were chromatographed on either vitronectin-Sepharose or Sepharose linked to the synthetic peptide Gly-Arg-Gly-Asp-Ser-Pro, which includes the fibronectin cell attachment sequence Arg-Gly-A...
Erkki Ruoslahti Engvall, E
Published in
Proceedings of the National Academy of Sciences
Alpha-fetoprotein (AFP), a fetal protein associated with certain tumors, and serum albumin did not corssreact immunologically in native form. Unfolding of their polypeptide chains by reduction of the disulfide bonds followed by carboxamidomethylation produced derivatives with immunochemical properties different from those of the native proteins. Pr...
Pierschbacher, Md Erkki Ruoslahti
Published in
Proceedings of the National Academy of Sciences
A tetrapeptide sequence, Arg-Gly-Asp-Ser, is the minimal structure recognized by cells in the large, adhesive glycoprotein fibronectin. We now have defined the structural requirements for this cell recognition site by testing several synthetic variants of the active tetrapeptide sequence. The conservative substitutions of lysine for arginine, alani...
